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KMID : 0603820080140010033
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Journal of Experimental & Biomedical Science
2008 Volume.14 No. 1 p.33 ~ p.38
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Antioxidant Effect of Citri Reticulatae Pericarpium Extract on Oxidative Stress-Mediated Cytotoxicity In Cultures
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Ha Dae-Ho
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Abstract
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In order to examine oxidative stress of reactive oxygen species and the antioxidant effect of Citri Reticulatae Pericarpium (CRP) extract, human skin melanoma cells were treated with various concentrations of hydrogen peroxide (H₂O₂). Antioxidant effect of CRP extract on H₂O₂-induced cytotoxicity, cell viability, DPPH-radical scavenging activity and superoxide dismutase (SOD)-like activity. In this study, H₂O₂ decreased cell viability of cultured human skin melanoma cells in dose- and time-dependent manners, and then, midcytotoxicity value (MCV) was determined at 60 ㎛ after human skin melanoma cells were cultured for 5 hours in the media containing ₂0~60 ㎛ of H₂O₂, respectively. The H₂O₂ was on cultured human skin melanoma cells because MCV of H₂O₂ was lower than 100 ㎛. In the antioxidant effect of CRP extract, CRP extract increased cell viability DPPH-radical scavenging activity and SOD-like activity. From these results, it is suggested that H₂O₂ was very toxic on cultured human skin melanoma cells. And also, CRP extract has the antioxidant effect on H₂O₂-induced cytotoxicity.
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KEYWORD
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Cytotoxicity, DPPH-radical scavenging activity, Citri Reticulatae Pericarpium
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